Journal: Immunity

Article Title: T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis

doi: 10.1016/j.immuni.2022.12.010

Figure Lengend Snippet: CD226 signaling is indispensable for IFNγ production from NK cells (A) Intravenous mouse model of 4T1 control or Pvr + metastasis in Il22 −/− mice. Animals received injections of anti-CD226 blocking antibody (420.1, 200 μg per mouse) or control i.p. on days 0, 3, and 14. (B) Numbers of macroscopic lung metastases and colonies in metastasis assay. (C) IFNγ-producing NK cells in the lungs evaluated by flow cytometry. Data presented as means ± SEM; p values <0.05 were considered significant by the Mann-Whitney U test. (D) Representative dot plots depict CD226 low, medium, and high NK cells. Frequency of IFNγ+ cells among NK cell populations (n = 5–6). Numbers represent the frequency of the parent gate. Data presented as means ± SEM; p values <0.05 were considered significant by the two-way ANOVA. (E) Intravenous mouse model of 4T1 control or Pvr + lung metastasis in Il22 −/− mice. Animals received injections of anti-TIGIT agonist antibody (1G9, 250 μg per mouse), anti-CD96 blocking antibody (3.3, 250 μg per mouse), or control i.p. on days 0, 3, and 14. (F) Numbers of macroscopic lung metastases. (G) IFNγ-producing NK and CD8 + T cells in the lungs were evaluated by flow cytometry. Numbers represent the frequency of the parent gate. Data presented as means ± SEM; p values <0.05 were considered significant by unpaired t- test (n = 5–6).

Article Snippet: InVivoMAb anti-mouse CD96 (3.3) , BioXCell , Cat# BE0337; RRID: AB_2894757.

Techniques: Control, Blocking Assay, Flow Cytometry, MANN-WHITNEY

Journal: Immunity

Article Title: T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis

doi: 10.1016/j.immuni.2022.12.010

Figure Lengend Snippet:

Article Snippet: InVivoMAb anti-mouse CD96 (3.3) , BioXCell , Cat# BE0337; RRID: AB_2894757.

Techniques: Purification, Control, Virus, Recombinant, Red Blood Cell Lysis, Transfection, Flow Cytometry, DNA Extraction, Gel Extraction, Plasmid Preparation, cDNA Synthesis, Cell Differentiation, Staining, Cell Isolation, Conjugation Assay, Sequencing, CRISPR, Retroviral, Software

Journal: PLoS ONE

Article Title: Interaction between nectin-1 and the human natural killer cell receptor CD96

doi: 10.1371/journal.pone.0212443

Figure Lengend Snippet: (A) Schematic representation of human CD96 protein, the 585 amino acid long isoform 2 is represented with residues numbered from methionine 1. The open box represents the signal peptide and the hatched box represents the transmembrane domain. The putative N-linked carbohydrates are shown as black lollipops. In the baculovirus construct, the mellitin signal peptide (black box) replaced the natural signal peptide (amino acids 1 to 21) from CD96. Human CD96 was truncated after lysine 516, and six histidine residues were added at the C terminus of hCD96(516t). (B) Silver stain and (C) western blot analysis of baculovirus-produced hCD96t after nickel chromatography purification and SDS-PAGE under denaturing and reducing conditions. Concentrations of imidazole used to elute proteins from the Ni-NTA-agarose column are indicated. FT = flow through. PBS indicates extensive PBS wash of the column before elution. Sizes of the molecular weight marker are indicated in kilodaltons. Anti-tetraHis Mab (QIAgen) was used to detect tagged hCD96t by western blot (C).

Article Snippet: The purified hCD96t was also detected by ELISA using a specific anti-CD96 monoclonal antibody (Clone 3H*, Abnova) (data not shown).

Techniques: Construct, Silver Staining, Western Blot, Produced, Chromatography, Purification, SDS Page, Molecular Weight, Marker

Journal: PLoS ONE

Article Title: Interaction between nectin-1 and the human natural killer cell receptor CD96

doi: 10.1371/journal.pone.0212443

Figure Lengend Snippet: Affinity and kinetics constants of CD96(517t) ectodomain to nectin-1(346t) ectodomain and nectin-1(143t) V-domain determined by SPR.

Article Snippet: The purified hCD96t was also detected by ELISA using a specific anti-CD96 monoclonal antibody (Clone 3H*, Abnova) (data not shown).

Techniques: